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negative control for mir-122 inhibitor anti-nc  (Ribobio co)

 
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    Ribobio co negative control for mir-122 inhibitor anti-nc
    Negative Control For Mir 122 Inhibitor Anti Nc, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/negative+control+(nc)+inhibitor/mir+122+inhibitor/pm40520367-220-25-31
    Average 90 stars, based on 1 article reviews
    negative control for mir-122 inhibitor anti-nc - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title:

    Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging


    Article Snippet: MicroRNA 4736 mimic and negative control (NC mimic), and miR-4736 inhibitors and negative control (NC inhibitor) were purchased from RiboBio Co, Ltd (Ribobio, Guangzhou, China) for upregulation and downregulation of miR-4736.



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    miR-208a-5p <t>inhibitor</t> rescues MSFs senescence induced by the silence of METTL3. (A) Cellular senescence was assessed via SA-β-gal staining in MSFs transfected with miR-208a-5p mimic for 48 h or co-treated with D-gal and miR-208a-5p inhibitor for 48 h (n=3). Scale bars: 100μm. **p < 0.01, ***p < 0.001. (B) Western blot analysis of P21 and P53 protein expression levels (n=3). **p < 0.01 vs. NC-mimic; ##p < 0.01 vs. NC-inhibitor + D-gal. (C) Scratch wound healing assay demonstrating miR-208a-5p’s effect on cell migration. Wound closure was quantified 12 h post-scratch (n=3). Scale bars, 100μm. **p < 0.01. (D) SA-β-gal staining showing senescence levels in MSFs co-transfected with METTL3 siRNA and miR-208a-5p inhibitor (n=3). Scale bars: 100μm. **p < 0.01 vs. NC-inhibitor; ##p < 0.01 vs. siMETTL3. (E) Proliferative capacity evaluated by EdU incorporation assay (n=3). Scale bars: 50μm. ***p < 0.001 vs. NC-inhibitor; ###p < 0.001 vs. siMETTL3. NC-mimic, <t>negative</t> <t>control</t> mimic; NC-inhibitor, negative control inhibitor.
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    negative control (nc) inhibitor  (Ribobio co)
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    miR-208a-5p <t>inhibitor</t> rescues MSFs senescence induced by the silence of METTL3. (A) Cellular senescence was assessed via SA-β-gal staining in MSFs transfected with miR-208a-5p mimic for 48 h or co-treated with D-gal and miR-208a-5p inhibitor for 48 h (n=3). Scale bars: 100μm. **p < 0.01, ***p < 0.001. (B) Western blot analysis of P21 and P53 protein expression levels (n=3). **p < 0.01 vs. NC-mimic; ##p < 0.01 vs. NC-inhibitor + D-gal. (C) Scratch wound healing assay demonstrating miR-208a-5p’s effect on cell migration. Wound closure was quantified 12 h post-scratch (n=3). Scale bars, 100μm. **p < 0.01. (D) SA-β-gal staining showing senescence levels in MSFs co-transfected with METTL3 siRNA and miR-208a-5p inhibitor (n=3). Scale bars: 100μm. **p < 0.01 vs. NC-inhibitor; ##p < 0.01 vs. siMETTL3. (E) Proliferative capacity evaluated by EdU incorporation assay (n=3). Scale bars: 50μm. ***p < 0.001 vs. NC-inhibitor; ###p < 0.001 vs. siMETTL3. NC-mimic, <t>negative</t> <t>control</t> mimic; NC-inhibitor, negative control inhibitor.
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    Image Search Results


    miR-208a-5p inhibitor rescues MSFs senescence induced by the silence of METTL3. (A) Cellular senescence was assessed via SA-β-gal staining in MSFs transfected with miR-208a-5p mimic for 48 h or co-treated with D-gal and miR-208a-5p inhibitor for 48 h (n=3). Scale bars: 100μm. **p < 0.01, ***p < 0.001. (B) Western blot analysis of P21 and P53 protein expression levels (n=3). **p < 0.01 vs. NC-mimic; ##p < 0.01 vs. NC-inhibitor + D-gal. (C) Scratch wound healing assay demonstrating miR-208a-5p’s effect on cell migration. Wound closure was quantified 12 h post-scratch (n=3). Scale bars, 100μm. **p < 0.01. (D) SA-β-gal staining showing senescence levels in MSFs co-transfected with METTL3 siRNA and miR-208a-5p inhibitor (n=3). Scale bars: 100μm. **p < 0.01 vs. NC-inhibitor; ##p < 0.01 vs. siMETTL3. (E) Proliferative capacity evaluated by EdU incorporation assay (n=3). Scale bars: 50μm. ***p < 0.001 vs. NC-inhibitor; ###p < 0.001 vs. siMETTL3. NC-mimic, negative control mimic; NC-inhibitor, negative control inhibitor.

    Journal: Frontiers in Immunology

    Article Title: METTL3-mediated m6A modification regulates D-galactose-induced skin fibroblast senescence through miR-208a-5p

    doi: 10.3389/fimmu.2025.1577783

    Figure Lengend Snippet: miR-208a-5p inhibitor rescues MSFs senescence induced by the silence of METTL3. (A) Cellular senescence was assessed via SA-β-gal staining in MSFs transfected with miR-208a-5p mimic for 48 h or co-treated with D-gal and miR-208a-5p inhibitor for 48 h (n=3). Scale bars: 100μm. **p < 0.01, ***p < 0.001. (B) Western blot analysis of P21 and P53 protein expression levels (n=3). **p < 0.01 vs. NC-mimic; ##p < 0.01 vs. NC-inhibitor + D-gal. (C) Scratch wound healing assay demonstrating miR-208a-5p’s effect on cell migration. Wound closure was quantified 12 h post-scratch (n=3). Scale bars, 100μm. **p < 0.01. (D) SA-β-gal staining showing senescence levels in MSFs co-transfected with METTL3 siRNA and miR-208a-5p inhibitor (n=3). Scale bars: 100μm. **p < 0.01 vs. NC-inhibitor; ##p < 0.01 vs. siMETTL3. (E) Proliferative capacity evaluated by EdU incorporation assay (n=3). Scale bars: 50μm. ***p < 0.001 vs. NC-inhibitor; ###p < 0.001 vs. siMETTL3. NC-mimic, negative control mimic; NC-inhibitor, negative control inhibitor.

    Article Snippet: A mimic negative control (NC-mimic) and an inhibitor negative control (NC-inhibitor), both sourced from RiboBio (Guangzhou, China), were utilized as the negative controls (NC).

    Techniques: Staining, Transfection, Western Blot, Expressing, Wound Healing Assay, Migration, Negative Control